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Kit for determination of the maturity of spermatozoan nucleoprotein (aniline blue staining method)
The kit is mainly used for detection of maturity of human spermatozoan nucleoprotein.
Product No.: BRED-013
Packing Specification: 40 tests/kitCategory： SpermFunc Kits → Diagnostic kits for sperm maturityIntroductionKit for Determination of the Maturity of Spermatozoan Nucleoprotein (Aniline Blue Staining Method)
In normal circumstances, the basic protein (nucleoprotein) bound to the sperm nucleus DNA will undergo the process
of natural maturity from the histone to protamine, of which, such substitute process of histone by protamine is called
nucleoprotein transition, which has an important physiological significance. The spermatozoid nucleus carries all
genetic information from paternal, and these genes must start the expression after the fertilization. Before the fertilization,
the sperm genes are specially protected by the protamine and closely concentrate with no DNA transcription. However,
the abnormality of nucleoprotein transition may cause the male infertility or early embryo abortion. The mechanism lies
in: a) the instability in sperm DNA and easily to be damaged and difficult to get pregnant; b) once fertilized, the
spermatozoid nucleus cannot normally depolymerized due to the nucleoprotein pattern, and thereby to influence the binding
of female and male pronuclei; c) the abnormal development of embryo may induce to the embryo abortion.
【Main compositions】
Solution A 1 vial 50ml
Solution B 1 vial 8.5ml
Solution C 1 vial 8.5ml
Solution D 3 vials 300ml 100ml/vial
Solution E 1 vial 10ml
Turbidity reference solution 1 vial 5ml
Tissue slide 10 pcs 8-well/pc
Cover slip 12 pcs
Slide barrel 1 pc
Instructions: The reagent is composed of aniline blue, acetic acid, phosphate buffer and preservative.
Each composition in kits in different lots can be used interchangeably.
【Storage conditions and duration】
1. Storage conditions
The kit should be kept out of sun and stored at the temperature of 2~8° C; the tissue slide, cover slip and slide barrel can
be stored at the room temperature.
2. Duration: The kit should be stored at the temperature of 2~8° C, with a use life of 12 months. Once unsealed, the kit
would be kept for 45 days at 2~8 ° C.
